Document Type : Research Paper


1 Ph.D. Student, Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran

2 Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran


To study the effect of  “cycloheximide” and amino acids of arginine and proline  on post-harvest longevity of cut Alstroemeria × hybrid ‘Mars’ flowers, a factorial experiment was conducted based on a randomized complete design with 3 replications and 20 treatments. The experiment includes CHI at 4 levels (0, 50, 100, 1000 µM), amino acid (AA) at 5 levels [0, 5, 10 mM (Arg) and 5, 10 mM proline] in terms of 24 h pulse. The results showed that flowers treated with “CHI50 x Pro10” had the most vase-life (9.3 days), water uptake (1.771 ml g-1 F.W.), and dry material (13.56 %), the least MDA aggregation (1.32 nmol g-1 F.W.), and the most CAT enzyme activity (5.68 nmol g-1 F.W. min-1). The treatment of “CHI10 x Arg5” was one of the most successful treatments in water uptake, wet weight, dry material, and total protein preservation, and MDA aggregation decrease, SOD enzyme activity increase, and took the second place in vase-life increase (9.08 days). Therefore, the 2 treatments of “CHI0 x Arg5” and “CHI50 x Pro10” are presented as the most successful treatments in quantitative and qualitative trait’s improvement of cut Alstroemeria flowers. The lowest positive impact on the majority of analyzed traits belongs to the treatment of 1000 µM CHI. Therefore, the application of high levels of CHI (1000 µM) on vase-life solution of cut Alstroemeria × hybrid ‘Mars’ is not recommended due to negative and toxic impact on post-harvest traits.

Graphical Abstract

Proline and Arginine Improves the Vase Life of Cut Alstroemeria‘Mars’ Flowers by Regulating Some Postharvest Physiochemical Parameters


Abdul-Qados, A.M.S. 2009. Effect of arginine on growth, yield and chemical constituents of wheat grown under salinity condition. Academic Journal of Plant Sciences, 2: 267-278.
Alia, P. and Saradhi, P. 1991. Proline accumulation under heavy metal stress. Journal of Plant Physiology, 138: 554-558.
Alipour, S., Farahmand, H. and Nasibi, F. 2013. Effect of different concentrations of arginine on vase life and antioxidant enzyme activity on cut tuberose (Polianthes tuberosa L.). 1st Symposium on New Discussion in Horticultural Science, Iran, Jahrom University, 264-267.
Alipour, S., Farahmand, H. and Nasibi, F. 2016. Effects of proline treatment on some morphological and physiological characteristics and increment of post-harvest life of tuberose cut flower (Polianthes tuberosa L.). Journal of Plant Process and Function, 4 (14): 105-114.
Alizadeh Matak, S., Hashemabadi, D. and Kaviani, B. 2017. Changes in postharvest physio-biochemical characteristics and antioxidant enzymes activity of cut Alsteroemeria aurantiaca flower as affected by cycloheximide, coconut water and 6-benzyladenine. Bioscience Journal, 33 (2): 321-332.
Amira, M.S. and Abdul, Q. 2010. Effect of arginine on growth, nutrient composition, yield and nutritional value of mung bean grown under salinity stress. Science and Nature, 8 (7): 30-41.
Aros, D., Suazo, M., Rivas, C., Zapata, P.,  Úbeda, C. and  Bridgen, M. 2019. Molecular and morphological characterization of new interspecific hybrids of Alstroemeria originated from A. caryophylleae scented lines. Euphytica, 215: 93.
Asadi Karam, E. and Asrar, Z. 2015. Allevation of oxidative damages induced by salinity in cress (Lepidium sativum) by pretreating with arginine. Journal of Crop Ecophysiology (Agriculture Science), 9 (33): 41 - 55.
Asghari, A., Razmjo, Zh. and Tehrani, M. 2006. Effect of nitrogen on yield and yield components and percentage of seed protein of four sorghum cultivars. Journal of Agricultural Science and Natural Reassures, 13: 45-54.
Beers, G.R. and Sizer, I.W. 1952. A spectrophotometric method for measuring the breakdown to hydrogen peroxide by catalase. Biological Chemistry, 195: 133-140.
Bidaki, S., Tehranifar, A. and Khorassani, R. 2018. Postharvest vase-life extension of fruits of two strawberry (Fragaria× ananassa Duch.) cultivars with amino acids application in soilless culture system. Journal of Science and Technology of Greenhouse Culture Soilless Culture Research Center, 9 (2): 1-10.
Borochov, A. and Woodson, W.R. 1989. Physiology and biochemistry of flower petal senescence. Horticultural Reviews, 11: 15-43.
Bouchereau, A., Aziz, A., Larher, F. and Martin-Tanguy, J. 1999. Polyamines and environmental challenges: Recent development. Journal of Plant Sciences, 140: 10-125.
Caputo, C. and Barneix, A. 1999. The relationship between suger and amino acid export to the pheloem in young wheat plant. Annals of Botany, 84: 33-38.
Chang, K.H., Wu, R.Y., Chuang, K.C., Hsieh, T.F. and Chung, R.S. 2010. Effects of chemical and organic fertilizers on the growth, flower quality and nutrient uptake of Anthurium andreanum, cultivated for cut flower production. Scientia Horticulturae, 125: 434-441.
Davies, A.1987. Protein damage and degradation by oxygen radicals. Journal of Biological Chemistry, 262: 9895-9901.
di Stasio, E., Rouphael, Y., Raimondi, G., El-Nakhel, C. and de Pascale, S. 2018. Postharvest performance of cut rose cv. Lovely Red as affected by osmo-protectant and antitranspirant compounds. Advances in Horticultural Science, 32 (3): 311-318.
Drory, A., Beja-Tal, S., Borochov, A., Gindin, E. and Mayak, S. 1995. Transient water stress in cut carnation flowers: Effects of cycloheximide. Scientia Horticulturae, 64 (3): 167-175.
Dubravina, G.A., Zaytseva, S.M. and Zagoskina, N.V. 2005. Changes in formation and localization of phenolic compounds in the tissues of European and Canadian yew during differentiation in vitro. Russian Journal of Plant Physiology, 52: 672-678.
Eason, J.R. and de Vré, L. 1995. Ethylene‐insensitive floralsenescence in Sandersonia aurantiaca (Hook.). New Zealand Journal of Crop and Horticultural Science, 23(4): 447-454.
Ezhilmathi, K., Singh, V.P., Arora, A. and Sairam, R.K. 2007. Effect of 5-sulfosalicylic acid on antioxidant activity in relation to vase life of Gladiolus cut flowers. Plant Growth Regulation, 51: 99–108.
Farshid, F., Hadavi, E. and Hekmati, J. 2013. Glutamine and malic acid increased the vase life of cut rose flowers (cv. Avalanch). Acta Horticulturae, 10 (12): 461-465.
Ferrante, A., Hunter, D.A., Hackett, W.P. and Reid, M. 2002. Thidiazuron a potent inhibitor of leaf senescence in Alestromeria. Postharvest Biology and Technology, 25: 333-338.
Ferrante, A., Mensuali-Sodi, A., Tognoni, F. and Serra, G. 2005. Postharvest studies on leaf yellowing of Chrysanthemum cut flowers. Advances in Horticultural Science, 19 (2): 81-85.
Galston, A.W. and Sawhney, R.K. 1990. Polyamines in plant physiology. Plant Physiology, 94: 404-410.
Geshnizjani, N. and Khosh-Khui, M. 2016. Promoted growth and improved quality of Gerbera jamesonni L. flowers using exogenous application of amino acids. International Journal of Horticultural Science and Technology, 3 (2): 155-166.
Giannopolitis, C. and Ries, S. 1997. Superoxid desmutase. I: Occurence in higher plant. Plant Physiology, 59: 309–314.
Gul, F. and Tahir, I. 2013. An effective protocol for improving vase life and postharvest performance of cut Narcissus tazetta flowers. Journal of the Saudi Society of Agricultural Sciences, 12: 39–46.
Gul, F., Tahir, I. and Rasool, I.U. 2012. Senescence and postharvest performance of cut Nerine sarniensis flowers: Effects of cycloheximide. International Journal of Botany, 8 (1): 22-30.
Gulzar, S., Tahir, I., Farooq, S. and Sultan, S.M. 2005. Effect of cytokinins on the senescence and longevity of isolated flowers of daylily (Hemerocallis fulva cv. "Royal Crown") sprayed with cycloheximide. Acta Horticulturae, 669: 395-403.
Haj Seyed Hadi, M.R., Darz, M.T., Ghandehari, Z. and Riazi, G. 2011. Effects of vermicompost and amino acids on the flower yield and essential oil production from Matricaria chamomile L. Journal of Medicinal Plants Research, 5(23): 5611-5617.
Heath, R.L. and Parker, L. 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stiochiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125: 189-198.
Islam, S.T., Tahir, I., Shahri, W. and Bhat, M.A. 2011. Effect of cycloheximide on senescence and postharvest performance in Hemerocallis fulva cv. Royal Crown. Journal of Plant Sciences, 6: 14-25.
Jones, R.B., Serek, M., Kuo, C.L. and Reid, M.S. 1994. The effect of protein synthesis inhibition on petal senescence in cut bulb flowers. American Society for Horticultural Science, 119: 1243-1247.
Kandil, M.M., El-Saady, M.B., Mona, H.M., Afaf, M.H. and Iman, M.E. 2011. Putrescine and uniconazole treatments on flower characters and photosynthetic pigments of Chrysanthemum indicum L. American Journal of Plant Sciences, 7: 399-408.
Karima, M., El-Din, G. and Abd El-Wahed, M.S.A. 2005. Effect of some amino acids on growth and essential oil content of chamomile plant. International Journal of Agriculture and Biology, 7: 376-380.
Khalil, S.I., El – Bassiouny, H.M.S., Hassanein, R.A., Mostafa, H.A.M., El – Khawas, S.A. and Abd El – Monem, A.A. 2009. Antioxidant defense system in heat shocked wheat plants previously treated with arginine or putrescine. Australian Journal of Basic and Applied Sciences, 1517-1526.
Kumar, N., Pal, M., Singh, A., Kumar Sairam, R. and Srivastava, G.C. 2010. Exogenous proline alleviates oxidative stress and increase vase life in rose (Rosa hybrida L. ‘Grand Gala’). Scientia Horticulturae, 127: 79-85.
Liu, J.H., Nada, K., Honda, C., Kitashiba, H. and Wen, X.P. 2006. Polyamine biosynthesis of apple callus under salt stress. Importance of the arginine decarboxylase pathway in stress responses. Journal of Experimental Botany, 57: 2589-2599.
Matysik, J., Alia, B., Halu, B. and Mohanty, P. 2002. Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Current Science Journal, 82: 525-532.
Mazumdar, B.C. and Majumder, K. 2003. Methods on physcochemical analysis of fruits. University College of Agriculture, Calcutta University, 136-150.
Mutui, T.M., Emongor, V.E. and Hutchinson, M.J. 2006. The effects of gibberellin4+7 on the vase life and flower quality of Alstroemeria cut flowers. Plant Growth Regulation, 48: 207-214.
Naghiloo, S., Soleimani, A., Rabiei, V., Khalighi, A. and Harkinezhad, M.T. 2020. Screening eight cultivars of Alstroemeria cut flower for vase life and biochemical traits. Journal of Ornamental Plants, 10 (2): 89-98.
Nasibi, F., Farahmand, H., Kamyab, A. and Alipour, S. 2014. Effects of arginine, cysteine and 5-sulfosalicylic acid on of vase life of tuberose cut flowers. Agricultural Communications, 2 (2): 35-41.
Nasibi, F., Kalantari, Kh.M., Mohammadinejad, Gh. and Zangeneh, R. 2016. Effects of amino acid arginine on some oxidative parameters and increase of salinity tolerance in wheat plant. Journal of Plant Research, 28 (5): 1119-1128.
Ortiz-Lopez, A., Chang, H.C. and Bush, D.R. 2000. Amino acid transporters in plants. Biochemistry and Biophysics, 1465: 275-280.
Pandey, S., Ranad, S.A., Nagar, P.K. and Kumar, N. 2000. Role of polyamines and ethylene as modulators of plant senescence. Journal of Biosciences, 25: 291-299.
Paulin, A. 1983. Improvement in the preservation of cut flowers. Acta Horticulturae, 138: 229-304.
Poursoltan Hojagan, M., Arouiee, H., Tabatabaei, S.J. and Neamati, S.H. 2017. Effect of amino acids foliar spraying on growth and physiological indices of tomato under salt stress conditions. Agroecology Journal, 13 (3): 41-50.
Rabiza-Świder R.J., Rybka, Z., Skutnik E. and Luka Szew Skaa, J. 2003. Proteolysis and expression of the cysteine protease gene in senescing cut leaves of Hosta ‘Undulata Erromena’ and Zantedeschia aethiopica Spr. treated with BA or GA3. Acta Physiologiae Plantarum, 25: 319-324.
Raymond, J., Rakariyatham, N. and Azanza, J.L. 1993. Purification and some properties of polyphenoloxidase from sunflower seeds. Phytochemistry, 34: 927–931.
Shahri, W. and Tahir, I. 2010. Effect of cycloheximide on senescence and postharvest performance of Ranunuculus asiaticus flowers. Pakistan Journal of Botany, 42 (5): 3577-3585.
Sultan, M. and Farooq, S. 1997. Effect of cycloheximide on some physiological changes associated with senescence of detached flowers of Iris germanica L. Acta Physiologiae Plantarum, 19 (1): 41-45.
Suttle, J.C. and Kende, H. 1980. Ethylene action and loss of membrane integrity during petal senescence in Tradescantia. Plant Physiology, 65: 1067-1072.
Szabados, L. and Savoure, A. 2009. Proline: A multifunctional amino acid. Trends in Plant Sciences, 15: 89-97.
Talaat, I.M., Bekheta, M.A., Mona, H. and Mahgoub, M. 2005. Physiological response of periwinkle plants (Catharanthus roseus L.) to tryptophan and putrescine. International Journal of Agriculture and Biology, 2: 210-213.
Thimann, K.V. 1987. Plant senescence: A proposed integration of the constituent processes page 1-19. In: Thomson, W.W., Northnegel, E.A. and Huffaker, R.C. (eds) plant senescence. Its biochemistry and physiology. American Society of Plant Physiologists, Rockvills Md.
Thomas, H. and Stodart, J.L. 1980. Leaf senescence. Annual Review of Plant Biology, 31: 83-111.
van Doorn, W.G., Harmannus, H. and Song, J.S. 1994. Water relations and senescence of cut Iris flowers: Effects of cycloheximide. Postharvest Biology and Technology, 5: 345-351.
van Meetren, U., van Iperen, W., Nijsee, J. and Keijzer, K. 2001. Processes and xylem anatomical properties involved in rehydration dynamics of cut flowers. Acta Horticulturae, 543: 207-211.
Wang, W., Gua, S., Chen, B., Huang, J. and Xing, J. 2017. Effect of postharvest L-arginine or cholesterol treatment on the quality of green asparagus (Asparagus officinalis L.) spears during low temperature storage. Scientia Horticulturae, 225: 788–794.
Wang, H.J., Wu, L.H., Wang, M.Y., Zhu, Y.H., Tao, Q.N. and Zhang, F.S. 2007. Effects of amino acids replacing nitrate on growth, nitrate accumulation, and macro element concentrations in pak-choi (Brassica chinensis L.). Pedosphere, 17: 595–600.
Wulster, G., Sacalis, J. and Janes, H. 1982. The effect of inhibitors of protein synthesis on ethylene induced senescence in isolated carnation petals. American Society for Horticultural Science, 107: 112–115.
Yaghoubi Kiaseh, D. and Yadegari, M. 2016. The effect of ethanol and cycloheximide on the vase life of cut flowers alstroemeria (Alstroemeria hybrida). Journal of Ornamental Plants, 6 (2): 73-82.
Yang, S.L., Lan, S.S. and Gong, M. 2009. Hydrogen peroxide-induced proline and metabolic pathway of its accumulation in maize seedlings. Plant Physiology, 166: 1694–1699.