Comparison of Plant Growth Regulators and Exogenous Ethylene Effects on Two Types of Cut Carnation (Dianthus caryophyllus L.)

Document Type: Original Article


1 Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Horticultural Sciences, Faculty of Agricultural Sciences and Natural Resources, University of Tehran, Karaj, Iran

3 Department of Forest, Research Institute of Forest and Rangelands, Tehran, Iran


The main object of this study is to investigate the effect of plant growth regulators on the prolongation of vase life of two kinds of cut carnation under normal and ethylene. Carnation is one of the most popular flowers that has a short life due to its sensitivity to ethylene. Also, the effect of plant growth regulators on several physiological traits under ethylene stress and normal conditions was studied. The effects of short-term treatment with benzyl adenine, salicylic acid, and methyl jasmonate on two types of cut carnations (Dianthus caryophyllus L.), one-headed standard cut carnation and multi- flower mini cut carnation, under exposure of ethylene condition were investigated. Benzyl adenine 100 µM short-term treatment had the most effect on total chlorophyll content. Salicylic acid 100 µM treatment enhanced the soluble carbohydrates, and also prolonged the cut carnation vase life. Methyl jasmonate 400 µM treating elevated the catalase and peroxidase enzymes activity and increased the proline content in cut carnations. All considered traits were reduced by exogenous ethylene. None of short-term treatments could repel the inappropriate exogenous ethylene effects. One-headed standard carnations were also tolerant of ethylene more than multi- flower mini carnations.

Graphical Abstract

Comparison of Plant Growth Regulators and Exogenous Ethylene Effects on Two Types of Cut Carnation (Dianthus caryophyllus L.)


  • It is recommended to use benzyl adenine, salicylic acid and methyl jasmonate to improve postharvest traits and extend the longevity of carnationcut flowers.


Alaey, M., Babalar, M., Naderi, R. and Kafi, M. 2011. Effect of pre and postharvest salicylic acid treatment on physio-chemical attributes in relation to vase-life of rose cut flowers. Postharvest Biology and Technology, 61: 91–94.

Alexopoulos, A.A., Akoumianakis, K.A., Vemmos, S.N. and Passam, H.C. 2007. The effect of postharvest application of gibberellic acid and benzyl adenine on the duration of dormancy of potatoes produced by plants grown from TPS. Postharvest Biology and Technology, 46: 54–62.

Arnon, A.N. 1967. Method of extraction of chlorophyll in the plants.
Agronomy Journal, 23: 112-121.

Bal, E. and Celik, S. 2010. The effects of postharvest treatments of salicylic acid and potassium permanganate on the storage of kiwifruit. Bulgarian Journal of Agricultural Science, 16 (5): 576-584.

Bates, L.S., Waldren, R.P. and Teare, I.D. 1973. Rapid determination of free proline for water stress studies. Plant and Soil, 39:  205-207.

Ben Hamed, K., Castagna, A., Salem, E., Ranieri, A. and Abdelly C. 2007. Sea fennel (Crithmum maritimum L.) under salinity conditions: A comparison of leaf and root antioxidant responses. Plant Growth Regulation, 53: 185–194.

Boxriker, M., Boehm, R., Krezdornc, N., Rotterc, B. and Piepho, H.P. 2017. Comparative transcriptome analysis of vase life and carnation type in Dianthus caryophyllus L. Scientia Horticulturae, 217: 61–72.

Comparot, S.M., Graham, C.M. and Reid, D.M. 2002. Methyl jasmonate elicits a differential antioxidant response in light and dark grown canola (Brassica napus) roots and shoots. Plant Growth Regulation, 38: 21-30.

Dias, M.A. and Costa, M.M. 1983. Effect of low salt concentrations on nitrate reductase and peroxidase of sugar beet leaves. Journal of Experimental Botany, 34: 537–543.

Ezhilmathi, K., Singh, V., Arora, A. and Sairam, R.K.  2007. Effect of 5-sulfosalicylic acid on antioxidant activity in relation to vase life of Gladiolus cut flowers. Plant Growth Regulation, 51 (2): 99-108.

Gapper, N.E., Coupe, S.A., Mckenzie, M.J., Sinclair, B.K., Lill, R.E. and Jameson, P.E.  2007. Regulation of harvest-induced senescence in broccoli (Brassica oleracea var. Italica) by cytokinin, ethylene, and sucrose. Plant Growth Regulation, 24: 153–16.

In, B.C., Strable, J., Binderc, B.M., Falbela, T.G. and Patterson, S.E. 2013. Morphological and molecular characterization of ethylene binding inhibition in carnations. Postharvest Biology and Technology, 86:  272–279.

Jung, S. 2004. Effect of chlorophyll reduction in Arabidopsis thaliana by methyl jasmonate or norflurazon on antioxidant systems. Journal of Plant Physiology and Biochemistry, 42:  231-255.

Jurgens, A., Witt, T. and Gottsberger, G. 2003. Flower scent composition in Dianthus and Saponaria species. Biochemical Systematics and Ecology, 31: 345-357.

Karimi, M., Hassanpour Asil, M., Nematzadeh, G. and Zakizadeh, H. 2012. Effects of anti-ethylene treatments on ethylene production and antioxidant activities in cut spray carnation.  Journal of Fruit and Ornamental Plant Research, 20: 173-182.

Kato, M. and Shimizu, S. 1987. Chlorophyll metabolism in higher plants. VII. Chlorophyll degradation in senescing tobacco leaves; phenolic-dependent peroxidative degradation. Canadian Journal of Botany, 65: 729–735.

Kazemi, M., Hadavi, E. and Hekmati, J. 2011. Role of salicylic acid in decreases of membrane senescence in cut carnation flowers. Journal of Agricultural Technology, 75:  1417-1425.

Khan, N.A., Shabian, S., Masood, A., Nazar, A. and Iqbal, N. 2010. Application of salicylic acid increases contents of nutrients and antioxidative metabolism in mungbean and alleviates adverse effects of salinity stress. International Journal of Plant Biology, 1: 1-8.

Kumari, G.J., Reddy, A.M., Naik, S.T., Kumar, S.G., Prasanthi, J., Sriranganayakulu, G., Reddy, P.C. and Sudhakar, C. 2006. Jasmonic acid induced changes in protein pattern, antioxidative enzyme activities and peroxidase isozymes in peanut seedlings. Biologia Plantarum, 50: 219-226.

Mansouri, H. 2012. Salicylic acid and sodium nitroprusside improve postharvest life of Chrysanthemums. Scientia Horticulturae, 145: 29–33.

Mei-Hua, F., Jian-Xin, W., Ge, S., Li-Na, S. and Rao-Fan, L. 2008. Salicylic acid and 6-BA affects in shelf-life improvement of Gerbera jamesonii cut flowers. Marine Science Technology, College of Zhejiang Ocean University, Zhoushan, Guangdong 316004, China Anhui Agricultural Science Bulletin, 8.

Munne-Bosch, S. and Alegre, L. 2004. Die and let live: Leaf senescence contributes to plants survival under drought stress. Functional Plant Biology, 31 (3): 203-216.

Schlegel, H.G. 1986. Die verwertung organischer säuren durch chlorella licht. Planta, 47 (5): 510-526.

Talla, S.K., Panigrahy, M., Kappara, S., Nirosha, P., Neelamraju, S. and Ramanan, R. 2016. Cytokinin delays dark-induced senescence in rice by maintaining the chlorophyll cycle and photosynthetic complexes. Journal of Experimental Botany, 67 (6): 1839–1851.

Varshney, A., Anis, M. and Aref, I.M. 2013. Potential role of cytokinin–auxin synergism, antioxidant enzymes activities and appraisal of genetic stability in Dianthus caryophyllus L. an important cut flower crop. In Vitro Cellular and Developmental Biology - Plant, 49: 166–174.

Wang, K., Jin, P., Cao, S., Shang, H., Yang, Z. and Zheng, Y. 2009. Methyl jasmonate reduces decay and enhances antioxidant capacity in Chinese bayberries. Journal of Agricultural and Food Chemistry, 57: 5809–5815.

Wasternack, C. and Kombrink, E. 2010. Jasmonates: Structural requirements for lipid-derived signals active in plant stress responses and development. Chemistry and Biology Journal, 5 (1): 63–77.

Xu, Y. and Huang, B. 2007. Heat-induced leaf senescence and hormonal changes for thermal bentgrass and turf-type bentgrass species differing in heat tolerance. Journal of the American Society for Horticultural Science, 132 (18): 2327-9788.

Yu, K.W., Gao, W., Hahnv, E.J. and Paek, K.Y. 2002. Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer. Biochemical Engineering Journal, 11: 211–215.

Zamani, S., Kazemi, M. and Aran, M. 2011. Postharvest life of cut rose flowers as affected by salicylic acid and glutamin. World Applied Sciences Journal, 12 (9): 1621-1624.