Micropropagation of Lantana camara Through Axillary Shoots Proliferation

Document Type: Original Article

Authors

1 Department of Horticultural Science, College of Agriculture, Urmia University, Orumieh, Iran.

2 Department of Horticultural Science, College of Agriculture, Isfahan University of Technology, Isfahan, Iran

Abstract

In vitro budinduction and shoot regeneration of Lantana camara L. was investigated using axillary nodal explants. Multiple shoot buds were induced on Murashige and Skoog (MS) media supplemented with 0, 2, 4 and 8 mg/l of 6-benzylaminopurine (BAP) or thidiazuron (TDZ) for proliferation and 0, 0.25 and 0.5 mg/l indole-3 butyric acid (IBA) for rooting. After 4 weeks, a maximum number of shoot buds was achieved on MS media containing 8 mg/l BAP and most buds were resulted in 8 mg/l TDZ. The results showed that the maximum length of shoots, internode length and weight were observed in MS media without any growth regulators. Suitable concentrations of cytokinins significantly increased RNA, DNA and protein synthesis and shoot induction. Difference in concentrations of BAP can be under the influence of genetic factors and experimental condition. Very low concentrations of TDZ stimulated the proliferation of lateral shoots in woody plants. Receptor molecules of TDZ had high affinity with this growth regulator. In this research, the highest number of roots was induced in MS media with 0.25 mg/l IBA. IBA is one of the strong PGRs in culture medium for stimulating and increasing the number of roots. IBA increases internal IAA, which is necessary for normal growth of root meristems and final development of roots. One of the effects of auxins is elongation of cells, but root cells need lower auxin for growth because auxin induces ethylene which is a root growth inhibitory.

Graphical Abstract

Micropropagation of <i>Lantana camara</i> Through Axillary Shoots Proliferation

Highlights

Axillary nodal explants of Lantana camara L. were proliferated in MS media containing 8 mg/l BAP and the highest number of roots was induced in MS media with 0.25 mg/l IBA.

Keywords


Abdellatef, E. and Khalafallah, M.M.  2007. Adventitious shoot formation and plant regeneration in medium staple cotton (Gossypium hirsitum L.) cv. ‘Barac B-67’. International Journal of Agriculture and Biology, 9 (6): 913- 916.

Abhyankar, G. and Reddy, V.D.  2007. Rapid micropropagation via axillary bud proliferation of Adhatoda vasica Nees. from nodal segments. Indian Journal of Experimental Biology, 45: 268-271.

Affonso, V.R., Bizzo, H.R., Lima, S.S., Esquibela, M.A. and Sato, A. 2007. Solid phase microextraction (SPME) analysis of volatile compounds produced by in vitro shoots of Lantana camara L. under the influence of auxins and cytokinins. Journal of the Brazilian Chemical Society, 18(8): 1504-1508.

Ahmad, N. and Anis, M. 2005. Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tissue and Organ Culture, 80: 187-190.

Ali, A., Ahmad, T., Abbasi, N.A. and Hafiz, A.A. 2009. Effect of different concenteration of auxins on in vitro rooting of olive cultivar ‘Moraiolo’. Pakistan Journal of Botany, 41(3): 1223-1231.

Ali Ahmed, A.B., Gouthaman, T., Rao, A.S. and Rao, M.V. 2005. Micropropagation of Phyla nodiflora (L.) Greene: An important medicinal plant. Iranian Journal of Biotechnology, 3(3): 186-190.

Amo-Marco, J.B. and Lledo, M.D. 1995. In vitro propagation of Salix tarraconensis Pau ex Font Quer. an endemic and threatened plant. In Vitro Cellular and Developmental Biology-Plant, 32: 42-46.

Ashok, K. and Bashir, J.M. 2010. In vitro propagation of a medicinal plant Portulaca grandiflora Hook. World Journal of Agricultural Science, 6(3): 327- 330.

Day, M.D., Wiley, C.J., Playford, J. and Zalucki, M.P. 2003. Lantana: current management status and future prospects for international agricultural research. Australian Centre International Agricultural Research, Canberra. 132 page.

Gaspar, T., Kevers, C., Faivre-Rampant, O., Crèvecoeur, M., Penel, C., Greppin, H. and Dommes, J. 2003. Changing concepts in plant hormone action. In Vitro Cellular and Developmental Biology-Plant, 39: 85–106.

Hiregoudar, L.V., Morthy, H.N., Bhat, J.G., Nayeen, A., Hema, B.P., Hahn, E.J. and Peak, K.Y. 2006. Rapid clonal propagation of Vitex trifolia. Biologia Plantarum, 50(2): 291-294.

Ismail, N., Rani, U., Sharma, N.N. and Batra, A. 2011. Influence of plant growth regulators on in vitro shoot regeneration via cotyledonary node in Clitoria ternatea L. International Journal of Pharmaceutical Sciences and Research, 2(3): 552-557.

Kesari, V., Ramesh, A.M. and Rangan, L. 2012. High frequency direct organogenesis and evaluation of genetic stability for in vitro regenerated Pongamia pinnata, a valuable biodiesel plant. Biomass and Bioenergy, 44: 23-32.

Khalafalla, M.M., Elaleem, K.G. and Modawi, R.S. 2010. Callus formation and organogenesis of potato (Solanum tuberosum L.) cultivar Almera. Journal of Phytology, 2: 40–46.

Kulkarni, A.A., Thengane, S.R. and Krishnamurthy, K.V. 2000. Direct shoot regeneration from node, internode, hypocotyl and embryo explants of Withania somnifera. Plant Cell Tissue and Organ Culture, 62: 203-209.

Lakshmi Prabha, A., Nandagopalan, V., Piramila, H.M. and Prabakaran, D.S. 2010. Standardization of in vitro studies on direct and indirect organogenesis of Trichosanthes cucumerina. Sixth International Plant Tissue Culture and Biotechnology Conferences. Plant Tissue Culture and Biotechnology, Pp: 73- 77.

Mao, A.A., Wetten, A., Fay, M.F. and Caligari, P.D.S. 2000. In vitro propagation of Litsea cubeba (Lours.) Pers. a multipurpose tree. Plant Cell Reports, 19: 263–267.

Mok, D.W.S. and Mok, M.C. 2001. Cytokinin metabolism and action. Plant Molecular Biology, 52: 89- 118.

Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiolia Plantarum, 15: 473-497.

Ning, G.G., Fan, X.L., Huang, W.J., Bao, M.Z. and Zhang, J.B. 2007. Micropropagation of six Prunus mume cultivars through axillary shoots proliferation and ISSR analysis of cloned plants. Acta Biologica Cracoviensia Series Botanica, 49(1): 25-31.

Nissen, S.J. and Sutter, E.G. 1990. Stability of IAA and IBA in nutrient medium to several tissue culture procedures. Horticultural Sciense, 25(7): 800-802.

Passos, J.L.,Meira, R.M.S.A.and Barbosa, L.C.A.2009.Foliar anatomy of the species Lantana camara and L. radula (Verbenaceae). Planta Daninha, 27 (4): 689-700.

Rai, V.R. 2002. Rapid clonal propagation of Nothapodytes foetida (Wight) Sleumer-Athreatened medicinal tree. In Vitro Cellular and Developmental Biology-Plant, 38 (4): 347-351.

Rathore, P., Suthar, R. and Purohi, S.D. 2008. Micropropagation of Terminalia bellerica Roxb. from juvenile explants. Indian Journal of Biotechnology, 7: 246-249.

Seetharma, Y.N., Rajana, L.N., Aravind, G.J.B., Sharanabasapa, G. and Mallikharjun, P.B. 2007. In vitro shoot regeneration from leaf and nodal explants of Veronina cinerea L. Indian Journal of Biotechnology, 6: 418-420.

Selvakumar, V., Anbudurai, P.R. and Balakumar, T. 2001. In vitro propagation of the medicinal plant Plumbago zeylanica L. through nodal explants. In Vitro Cellular and Developmental Biology-Plant, 37: 280-284.

Sharma, R. and Shahzad, A. 2008. Thidiazuran (TDZ) induced regeneration from cotyledonary node explant of Abelmoschus moschatus Medik. L., (A valuable medicinal plant). World Journal of Agricultural Sciences, 4(4): 449-452.

Singh, J. and Tiwari, K.N. 2010. High-frequency in vitro multiplication system for commercial propagation of pharmaceutically important Clitoria ternatea L., A valuable medicinal plant. Industrial Crops and Products, 32: 534–538.

Srivastava, P., Sisodia, V. and Chaturvedi, R. 2011. Effect of culture conditions on synthesis of triterpenoids in suspension cultures of Lantana camara L. Bioprocess and Biosystematic Engineering, 34: 75–80.

Stefancic, M., Stampar, F. and Osterc, G. 2005. Influence of IAA and IBA on root development and quality of Prunus GiSelA5 leafy cutting. Horticultural Science, 40(7): 2052-2055.

Te-chato, S. and Lim, M. 2000. Improvement of mangosteen micropropagation through meristematic nodular callus formation from in vitro-derived leaf explants. Scientia Horticulturae, 86: 291-298.

Verma, S.K., Yucesan, B.B., Sahin, G., Gurel, S. and Gurel, E. 2011. Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species. Turkish Journal of Botany, 35: 689-695.

Victor, J.M.R., Murthy, B.N.S., Murch, S.J. Krishnaraj, S. and Saxena, P. 1999. Studies of endogenous purine metabolism in thidiazuron-induced somatic embryogenesis of peanut (Arachis hypogea L.). Plant Growth Regulation, 28: 41- 47.

Wahab, F., Nabi, Gh., Ali, N. and Shah, M. 2001. Rooting response of semi-hard wood cutting of guava (Psidium guajava L.) to various concentrations of different auxins. Online Journal of Biological Sciences, 1(4): 184-187.

Widiyanto, S.N., Erytrina, D. and Rahmania, H. 2005. Adventitious shoot formation on teak (Tectona grandis L.) callus cultures derived from internodal segments. IInd IS on Biotechnology of Tropical and Subtropical Species. Pp. 153-157.

Zulfiqar, B., Abbasi, N.A., Ahmad, T. and Hafiz, I.A. 2009. Effect of explant sources and different concentrations of plant growth regulators on in vitro shoot proliferation and rooting of avocado (Persea americana Mill.) cv. “Fuerte”. Pakistan Journal of Botany, 41(5): 2333- 2346.