Effect of BAP and NAA on Micropropagation of Caladium bicolor (Aiton) Vent., an Ornamental Plant

Document Type: Original Article

Authors

1 Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran

2 Master of Science and Ph.D. Student, Hyrcan Agricultural Sciences and Biotechnology Research Institute, Amol, Iran

3 Master of Science, Hyrcan Agricultural Sciences and Biotechnology Research Institute, Amol, Iran

4 Department of Agronomy, Rasht Branch, Islamic Azad University, Rasht, Iran

Abstract

Leaf explants of kaladium dwubarwne Caladium bicolor (Aiton) Vent., were cultured in vitro on MS media supplemented with 25 different concentrations of BAP and NAA in order to determine the appropriate concentrations for micropropagation. All combinations induced callus formation on explants. Callus production on leaves explants grown on control medium was very low. The medium enriched with 4 mg l-1 BAP + 0.5 mg l-1 NAA was the most effective for callus formation. The highest number of shoots (6.43 per explant) and roots (5.56 per explant) were regenerated on media containing 1 mg l-1 BAP + 0.5 mg l-1 NAA and 3 mg l-1 BAP + 0.5 mg l-1 NAA, respectively. The regenerated plantlets were grown in a greenhouse and acclimatized successfully.

Keywords


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