Spatial Distribution Pattern of Tetranychus urticae Koch (Acari: Tetranychidae) on Different Rosa Cultivars in Greenhouse Tehran

Document Type: Original Article


1 Institute of Agricultural Science, University of Zabol, Zabol, Iran

2 Department of Entomology, Faculty of Agriculture, Islamic Azad University, Arak .Iran.

3 Ph.D. Student in Agricultural Entomology Arak, Arak Iran


The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is an economically important pest of ornamental plant in Iran. The population abundance and spatial distribution were studied on five Rosa cultivars including Maroussia, Wendela, Elderado, Wenedetta, and Hot Lady during two growing seasons of 2011 and 2012 in greenhouse. The k parameter, index of dispersion, Lloyd’s mean crowding, Morisita’s index and regression methods (Taylor and Iwao) were used to estimate the spatial distribution pattern of mite. There was improved sampling program by k parameter and coefficients of regression models. The result indicated that the highest population density of T. urticae was early of July (2.47 per leaf) and early of September (4.11 per leaf). Also, a sequential sampling plan was developed using the fixed-precision method of Green for estimating the density of the mite. Populations on different
Rosa cultivars, especially Maroussia, were aggregated during most of the growing season and negative binomial models fit data sets better than the Poisson series. The mean number of two-spotted spider mite per plant had significantly
difference between all plant strata. The fitness percentage of cultivar Maroussia Rosa revealed a slight tendency towards the Poisson distribution, so that 58 and 14.1 percent of data sets from T. urticae on cultivar Wenedetta
populations fit the Poisson distribution. These results fully support findings of distribution indices, indicating an aggregated distribution. Determining sampling program and spatial distribution pattern of the pest can effectively help to design and perform of IPM.


Ahmadi, M., Fathipur, Y. and Kamali. K. 2005. Population density and spatial distribution of Tetranychus
urticae Koch (Acari: Tetranychidae) on different bean cultivars in Tehran region. Iranian
Journal of Agricultural Sciences, 36: 1087-1092.
Demirozer, O. 2012. First record of the cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera:

Noctuidae), on the oil-bearing rose, Rosa damascenaMiller, in Turkey. Hellenic Plant Protection
Journal 5: 27-29.
Feng, M.G., Nowierski, R.M., Zeng, Z. and Scharen, A.L. 1993. Estimation of population density
of the Russian wheat aphid (Homoptera: Aphididae) from the proportion of grain tillers
with the different tally threshold of aphids. Journal of Economic Entomology. 86: 427-435.
Grbic, M., Van Leeuwen, T., Clark, R.M., Rombauts, S., Rouze, P., Grbic, V., Osborne, E.J., Dermauw,
W. and Ngoc, P.C. 2011. The genome of Tetranychus urticae reveals herbivorous pest adaptations.
Bulletin of the Entolomological Society of Egypt. LVIII: 249-259.
Greco, N. M., Liljesthrom, G. G. and Sanchez, N. E. 1999. Spatial distribution and coincidence of
Neoseiulus californicus and Tetranychus urticae (Acari: Phytoseiidae, Tetranychidae) on
strawberry. Journal of Experimental and Applied Acarology, 23: 567-580.
Green, R. H. 1970. On fixed precision level sequential sampling. Research in Population Ecology,
Greig-Smith, P. 1952. The use of random and contiguous quadrates in the study of the structure of
plant communities. Annals of Botany, 16: 293–316.
Hassell, M.P. and May, R.M. 1974. Aggregation of predators and insect parasites and its effect on
stability. Journal of Animal Ecology, 43: 567–94.
Hillhouse, T.L. and Pitre, H.N. 1974. Comparison of miner Liriomyza sativae Blanchard in Khuzestan
sampling techniques to obtain measurements of insect populations on soybeans province.
Journal of Economic Entomology, pp: 251. 67: 411-414.
Iwao, S. and Kuno, E. 1968. Use of the regression of mean crowding on mean density for estimating
sample size and the transformation of data for the analysis of variance. Research in Population
Ecology, 10: 210.
Kogan, M. and Herzog, D. C. 1980. Sampling methods in soybean entomology. Springer Verlag,
New York, 587 PP.
Kuno, E. 1991 Sampling and analysis of insect populations. Annual Review of Entomology, 36: 285-304.
Mehrkhou, F., Fathipour, Y., Talebi, A. A., Kamali, K. and Naseri, B. 2008. Population density and
spatial distribution patterns of Tetranychus urticae (Acari: Tetranychidae) and its predator
Stethorus gilvifrons (Col.: Coccinellidae) on different crops in Tehran area. Journal of Entomology
Research Society, 10(2): 23-36.
Pakyari, .2012. Spatial distribution pattern of Tetranychus urticae and its egg predator Scolothrips
longicornis on different bean cultivars. Munis Entomology and Zoology. 7(1): 243-255.
Pedigo, L.P. 1994. Introduction to sampling arthropod populations, In: L.P. Pedigo and G.D. Buntin,
(eds) Handbook of sampling methods Entomology, 32: 201-224.
Pedigo, L. P. and Buntin, G. D. 1994. Handbook of sampling methods for arthropods in agriculture.
CRC Press, Florida, 714 PP.
Sedaratian, A., Fathipour, Y., Moharramipour, S. and Talebi, A. A. 2008. Effect of different soybean
cultivars on bionomics of Tetranychus urticae (Acari: Tetranychidae). Munis Entomology
and Zoology, 3 (2): 716-730.
Slone, D. H. and Croft, B. A. 1998. Spatial aggregation of apple mites (Acari: Phytoseiidae, Stigmaeidae,
Tetranychidae) as measured by a binomial model: effects of life stage, reproduction, competition
and predation. Environmental Entomology, 27: 918-925.
Southwood, T.R.E. 1995. Ecological methods with particular reference to the study of insect populations.
Chapman and Hall, London. 524 PP.
Southwood, T. R. E. and Henderson, P. A. 2000. Ecological methods. 3rd edition. Blackwell Sciences,
Oxford, 592 PP.
Takahashi, H., Takafuji, A., Takabayashi, J., Yano, S. and Shimoda, T. 2001. Seasonal occurrence
of specialist and generalist insect predators of spider mites and their response to volatiles
from spider-mite-infested plants in Japanese pear orchards. Experimental and Applied Acarology,
25: 393-402.

Taylor, L.R. 1986. Synoptic dynamics, migration and the Rothamsted Insect Survey. Journal of Animal
Ecology 55: 1–38.
Yan, C. 1998. Spatial pattern and vertical distribution of vegetable leaf miner Liriomyza sativae
Blanched (Diptera: Agromyzidae). Journal of Fujian Agricultural University. Doi: CNKI:
SUN: FJND.0.1998-03-013.
Young, L.J. and Young, L.H. 1998. Statistical ecology. Kluwer Academic Pub Boston, pp: 565.
Zenjun, S. 1997. Spatial distribution pattern and sampling methods on Liriomyza sativae. Guizhou.
Agricultural Science. DOI: CNKI: ISSN: 1001-3601.0.1997-05-007.