Department of Botany, University of Delhi’ Delhi – 110007, India
Plant tissue culture refers to growing and multiplication of cells, tissues and organs of plants on defined solid or liquid media under aseptic and controlled environment. Micropropagation allows rapid production of high quality, disease-free and uniform planting material. The micropropagation of high quality planting materials of ornamentals, and forest and fruit trees has created new opportunities in global trading for producers, farmers, and nursery owners, and for rural employment. A substantial number of micropropagated plants do not survive transfer from in vitro conditions to greenhouse or field environments. The greenhouse and field have substantially lower relative
humidity, higher light level and septic environment that are stressful to micro-propagated plants compared to in vitro conditions. It is possible to acclimatize plantlets during in vitro as well as in vivo by various methods and thereby fa-cilitating the successful transfer of in vitro cultured plantlets to soil.