Evaluation of Main Anthocyanins in Skin of Forest Pear (Pyrus pyraster) (Sange Khouj) Fruit in Guilan Province of Iran


1 Young Researchers Club, Shahre-Qods Branch, Islamic Azad University, Tehran, Iran

2 Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran


The anthocyanins of the fruit skin of Iranian forest pear (Sange Khouj) were separated, identified and quantified using high-performance liquid chromatography/diode array detection (HPLC/DAD) and HPLC/electrospray ionization /mass spectrometry (HPLC/ESI /MS). The individual anthocyanins were identified by comparing their mass spectral data and retention times with those of standards and published data. Cyanidin-3-galactoside was the major compound, taking up 93% of the total anthocyanin content. The content of Cyanidin-3-galactoside reached 23.7±3.2 mg 100 g-1 fresh weight, followed by the eighth peak (2.14%) and Cyanidin-3-glucoside (1.23%). Pelargonidin- 3-rutinoside was identified for the first time in ordinary pear (P. communis L). DPPH (2, 2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing antioxidant power) assays showed that the extract from the fruit peel of ‘Sange Khouj’ contained anthocyanins and possessed high antioxidant capacity.