Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran


In the present study, 2, 4-dichlorophenoxyacetic acid (2, 4-D) was assessed individually for its effectiveness to induce somatic embryogenesis in tea (Camellia sinenesis L.). Embryonic axes and cotyledons explants were dissected from the seeds. Explants were cultured on Murashige and Skoog (MS) medium containing 0, 1 and 5 mM 2, 4-D alone for embryonic axes and 0, 1 and 5 mM 2, 4-D along with 0 and 0.5 mM IBA for cotyledons. Embryos were observed in embryonic axes explants cultured on MS medium containing 1mM 2, 4-D. No somatic embryos were seen on cotyledons explants.