Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran
Shoot tips from actively growing, greenhouse maintained plants of Kalanchoe blossfeldiana were cultured in vitro for shoot proliferation and root initiation on Murashige and Skoog (MS) basal medium supplemented with NAA and BA, both in concentrations of 0.00, 0.50, 1.00 and 2.00 mg l-1. Results showed that the maximum plantlets height (7.012 cm), node number (4.516), root number (8.860) and root length (10.160 cm) were obtained in MS medium containing 1 mg l-1 BA + 1 mg l-1 NAA. Maximum shoot number (5.886), leaf number (8.980) and proliferation index (1.791) were calculated in medium supplemented with 1 mg l-1 BA + 0.5 mg l-1 NAA. Minimum plantlets height (1.988 cm), node number (1.283), root number (2.720), root length (3.016 cm), shoot number (1.221), leaf number (2.015) and proliferation index (0.405) were obtained in medium without BA and NAA (control). Fresh and dry weights of plantlets were calculated, too. About 85% of the micropropagated plantlets were established successfully in acclimatization medium containing peat, perlite and sand (1:1:1). Regenerated plantlets were morphologically identical with mother plants.