1 Department of Horticulture, Faculty of Agriculture Hajee Mohammad Danesh Science and Technology University Dinajpur 5200, Bangladesh Greenhouse Laboratory Center Technische Universität München Dürnast 3, D-85354 Freising, Germany

2 Hochschule Weihenstephan-Triesdorf Fakultät Gartenbau und Leben smittel Technologie Am Staudengarten 8 D-85354 Freising, Germany


The influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘Bianca’ and ‘El Torro’. Third, fourth and fifth nodal explants were cultured on the modified MS medium supplemented with 1.0 and 5.0 mg/l BA. In both the cultivars, higher rate of proliferation (‘Bianca’ 3.75; ‘El Torro’ 2.65) were obtained from the explants distal to the apex than those of the proximal position with 5.0 mg/l BA. But proliferating shoots derived from the fifth nodal explant with 1.0 mg/l attained highest shoot length (‘Bianca’ 1.43 cm; ‘El Torro’ 1.19 cm) and produced higher number of leaves (‘Bianca’ 5.45; ‘El Torro’ 6.30) and fresh weight (‘Bianca’ 659.38 mg; ‘El Torro’ 255.95 mg) per explant than the third and fourth nodal explants. The fifth nodal explant with 1.0 mg/l BA was found the best treatment for the shoot regeneration of rose cvs. ‘Bianca’ and ‘El Torro’.


1. Anonymous. 2009. In vitro produktion der laboratorien des ADIVK. ADIVK-aktuell. 13(2):10-16.
2. Anonymous. 2013. Landwirschaftliche bodennutzung - anbau von zierpflanzen 2012. Statistisches Bundensamt, Wiesbaden 2013.
3. Ara, K.A., Hossain, M.M., Qusim, M.A., Ali, M. and Ahmed, J.U. 1997. Micropropagation of rose (Rosa sp. cv. Peace). Plant Tissue Culture. 7: 135-142.
4. Asadi, A.A., Vedadi, C., Rahimi, M. and Naserian, B. 2009. Effect of plant growth hormones on root and shoot regeneration in Rose 'Morrasia' under in vitro conditions. Bioscience Research. 6(1): 40-45.
5. Bressan, P.H., Kim, Y.J., Hyndman, E., Hasegawa, P.M. and Bressan, R.A. 1982. Factors affecting in vitro propagation of rose. Journal of the American Society for Horticultural Science. 107: 979-990.
6. Carelli, B.P. and Echeverrigaray, S. 2002. An improved system for the in vitro propagation of rose cultivars. Scientia Horticulturae. 92: 69-74. [DOI via Crossref]   
7. Davies, D.R. 1980. Rapid propagation of roses in vitro. Scientia Horticulturae. 13: 385- 389. [DOI via Crossref]   
8. Hameed, N., Shabbir, A., Ali, A. and Bajwa, R. 2006. In vitro micropropagation of disease free rose (Rosa indica L.). Mycopathology. 4(2): 35-38.
9. Hisa, C.N. and Korban, S.S. 1996. Factors affecting in vitro establishment and shoot proliferation of Rosa hybrid L. and Rosa chinensis minima. In vitro Cellular and Development Biology. 32: 217-222. [DOI via Crossref]   
10. Horn, W., Schlegel, G. and Hauft, B. 1988. Micropropagation of roses. Acta Horticulturae. 226: 623-626.
11. Horn, W. 1992. Micropropagation of rose (Rosa sp. L.). In: BAJAJ YPS. (Ed.). Biotechnology in Agriculture and Forestry 20 - High-Tech and Micropropagation. Berlin: Springer-Verlag,320-340.
12. Kim, C.K., Oh, J.Y., Jee, S.O. and Chung, J.D. 2003. In vitro micropropagation of Rosa hybrid L. Journal of Plant Biotechnology. 5: 115-119.
13. Ma, Y., David, H.B. and Chen, J. 1996. Propagation of rose species in vitro. In vitro Cellular and Development Biology. 32: 103-108. [DOI via Crossref]   
14. Marcelis-van Acker, C.A.M. and Scholten, H.J. 1995. Development of axillary buds of rose in vitro.Scientia Horticulturae. 63: 47-55. [DOI via Crossref]   
15. Misra, P. and Chakrabarty, D. 2009. Clonal propagation of Rosa clinophylla Thory. through axillary bud culture. Scientia Horticulturae. 119: 212-216. [DOI via Crossref]   
16. Podwyszynska, M. and Olszewski, T. 1995. Influence of gelling agents on shoot multiplication and the uptake of macroelements by in vitro culture of rose, cordyline and Homalomena. Scientia Horticulturae. 64: 77– 84. [DOI via Crossref]   
17. Razavizadeh, R. and Ehsanpour, A.A. 2008. Optimization of in vitro propagation of Rosa hybrid L. cultivar 'Black Red'. American-Eurasian Journal of Agriculture and Environment Science. 3(1): 96-99.
18. Rout, G.R., Samantaray, S., Mottley, J. and Das. P. 1999. Biotechnology of the rose: a review of recent progress. Scientia Horticulturae. 81: 201-228. [DOI via Crossref]   
19. Salehi, H. and Khosh-Khui, M. 1997. Effects of explant length and diameter on in vitro shoot growth and proliferation rate of miniature roses. Journal of Horticultural Science. 72: 673-676.
20. STSC, Inc. 1987. STATGRAFICS Users' Guide.
21. Vijaya, N., Satyanarayana, G., Prakash, J. and Pierik, R.L.M. 1991. Effect of culture media and growth regulators on in vitro propagation of rose. Current Plant Science and Biotechnology in Agriculture. 12: 209-214. [DOI via Crossref]   
22. Waseem, K., Jilani, M.S. and Khan, M.S. 2009. Rapid plant regeneration of chrysanthemum (Chrysanthemum morifolium L.) through shoot tip culture. African Journal of Biotechnology. 8(9): 1871-77.
23. Yakimova, E., Kapchina-Toteva, V., Groshkoff, I. Mandivanova, G. 2000. Effect of BA and CPPU on protease and α-amylase activity of in vitro cultured explants of Rosa hybrid L. Bulgarian Journal of Plant Physiology. 26(1-2): 39-47.