Plantlet Regeneration through Indirect Organogenesis of Flame Gold Tree (Koelreuteria elegans Laxm.)

Authors

1 Department of Botany, Kurukshetra University, Kurukshetra 136119 (India)

2 Principal, Govt. Degree College, Hiranagar, Kathua

Abstract

Koelreuteria elegans, popularly known as “Flame Gold” is an ornamental tree. In vitro callus induction and regeneration from various explants (eaf segments and cotyledonary leaf) were studied on modified MS medium. The highest callus induction rate (80%) and multiplication was obtained in 2 mg/l 2,4-D from leaf segments. Calli transferred in 1.5 mg/l BAP resulted in efficient shoot regeneration (70%) and development (4.35 shoots). MS half strength medium supplemented with 0.2 mg/l NAA reported 80% rooting after 21 days of implantation. Mostly, the roots were long and healthy. Plants were successfully transferred in sterilized mixture of vermiculite: soil: sand (3:1:1) with 65% survival rate under field conditions. The in vitro regenerated plantlets were hardened and acclimatized successfully.

Keywords


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