Some Useful Information about Micropropagation


Department of Horticultural Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran


This review article investigates a comprehensive description of the factors, methods, strategies, approaches and prerequisites influencing in vitro micropaopagation and the amount of their use across the world. The findings presented here are the results of the study of several hundred papers published all over the world. Tissue culture techniques are routinely used for micropropagation. Enhancement of efficiency and efficacy of plant regeneration are primary goals of micropropagation. In this article, maximum and minimum use of some basic issues concerned with plant tissue culture in vitro especially micropropagation such as types of explants, types of culture media, types of sterilizing agent for explants, types of plant growth regulators (PGRs) for general studies of miro-propagation, shoot and root induction, somatic embryogenesis (SE) and callus induction have been considered. Maximal application for explants, culture media and sterilizing agent are single node, Murashige and Skoog (MS) and sodium hypochlorite (NAClO), respectively. BA and NAA are the most application among cytokinins (CKs) and auxins for general studies of miroprop-agation. Among all PGRs used for general studies of miropropagation, NAA is on the top. BA and IBA are the most use among CKs and auxins for shooting and rooting of explants, respectively. 2,4-D, NAA and TDZ are used more than the other PGRs for induction of SE. Among all types of auxins and CKs used as singular or in combination with them for callus induction, 2,4-D is at the top level. Combination of BA and NAA is the maximum for general studies of miropropagation. This review article can help to the future studies on micro-propagation due to the correct selection of the treatments