Behzad Kaviani
Volume 5, Issue 1 , Autumn 1393, , Pages 29-40
Abstract
This review article investigates a comprehensive description of the factors, methods, strategies, approaches and prerequisites influencing in vitro micropaopagation and the amount of their use across the world. The findings presented here are the results of the study of several hundred papers published ...
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This review article investigates a comprehensive description of the factors, methods, strategies, approaches and prerequisites influencing in vitro micropaopagation and the amount of their use across the world. The findings presented here are the results of the study of several hundred papers published all over the world. Tissue culture techniques are routinely used for micropropagation. Enhancement of efficiency and efficacy of plant regeneration are primary goals of micropropagation. In this article, maximum and minimum use of some basic issues concerned with plant tissue culture in vitro especially micropropagation such as types of explants, types of culture media, types of sterilizing agent for explants, types of plant growth regulators (PGRs) for general studies of miro-propagation, shoot and root induction, somatic embryogenesis (SE) and callus induction have been considered. Maximal application for explants, culture media and sterilizing agent are single node, Murashige and Skoog (MS) and sodium hypochlorite (NAClO), respectively. BA and NAA are the most application among cytokinins (CKs) and auxins for general studies of miroprop-agation. Among all PGRs used for general studies of miropropagation, NAA is on the top. BA and IBA are the most use among CKs and auxins for shooting and rooting of explants, respectively. 2,4-D, NAA and TDZ are used more than the other PGRs for induction of SE. Among all types of auxins and CKs used as singular or in combination with them for callus induction, 2,4-D is at the top level. Combination of BA and NAA is the maximum for general studies of miropropagation. This review article can help to the future studies on micro-propagation due to the correct selection of the treatments
Zahra Bagheri Tirtashi; Davood Hashemabadi; Behzad Kaviani; Ameneh Sajjadi; Maryam Jadidsolymandarabi
Volume 4, Issue 3 , Spring 1393, , Pages 163-168
Abstract
In this research, the effects of thidiazuron pulse treatment and salicylic acid were examined to improve vase life and maintain the quality of Alstroemeria ҅Modena҆ cut flowers. The experiment was done in a factorial experiment based on RCD with 16 treatments, 3 replications and 48 plots. The flowers ...
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In this research, the effects of thidiazuron pulse treatment and salicylic acid were examined to improve vase life and maintain the quality of Alstroemeria ҅Modena҆ cut flowers. The experiment was done in a factorial experiment based on RCD with 16 treatments, 3 replications and 48 plots. The flowers were placed in different concentrations of thidiazuron (0, 10, 20, and 50 μM) and salicylic acid (0, 100, 200, and 300 mg l-1) for 24 hours. Then cut flowers were put in a preservative solution containing 3% sucrose and 300 mg l-1 8-HQS.Then, vase life and quality traits such as fresh weight, dry weight, water uptake, amount of soluble solids (˚brix) and cell membrane stability (electrolyte leakage) were evaluated during examination. The results showed that the concentration of 200 mg l-1 salicylic acid, has the highest water uptake and lowest reduction of fresh weight in comparison with the other treatments. In all treatments except for the control, dry weight and soluble solids increased.Also, 20 μM thidiazuron and 100 mg l-1 salicylic acid showed the greatest stability of the cell membrane compared to the control treatment. Finally, 20 μM thidiazuron and 200 mg l-1 salicylic acid with the highest vase life of cut alstroemeria ҅Modena҆ compared to the other treatments is recommended to extend the vase life.
Behzad Kaviani; Davood Hashemabadi; Mohaddeseh Kordi
Volume 4, Issue 2 , Winter 1393, , Pages 101-106
Abstract
Shoot tips from actively growing, greenhouse maintained plants of Kalanchoe blossfeldiana were cultured in vitro for shoot proliferation and root initiation on Murashige and Skoog (MS) basal medium supplemented with NAA and BA, both in concentrations of 0.00, 0.50, 1.00 and 2.00 mg l-1. Results showed ...
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Shoot tips from actively growing, greenhouse maintained plants of Kalanchoe blossfeldiana were cultured in vitro for shoot proliferation and root initiation on Murashige and Skoog (MS) basal medium supplemented with NAA and BA, both in concentrations of 0.00, 0.50, 1.00 and 2.00 mg l-1. Results showed that the maximum plantlets height (7.012 cm), node number (4.516), root number (8.860) and root length (10.160 cm) were obtained in MS medium containing 1 mg l-1 BA + 1 mg l-1 NAA. Maximum shoot number (5.886), leaf number (8.980) and proliferation index (1.791) were calculated in medium supplemented with 1 mg l-1 BA + 0.5 mg l-1 NAA. Minimum plantlets height (1.988 cm), node number (1.283), root number (2.720), root length (3.016 cm), shoot number (1.221), leaf number (2.015) and proliferation index (0.405) were obtained in medium without BA and NAA (control). Fresh and dry weights of plantlets were calculated, too. About 85% of the micropropagated plantlets were established successfully in acclimatization medium containing peat, perlite and sand (1:1:1). Regenerated plantlets were morphologically identical with mother plants.
Shahram Shoa Kazemi; Davood Hashemabadi; Ali Mohammadi Torkashvand; Behzad Kaviani
Volume 4, Issue 2 , Winter 1393, , Pages 107-114
Abstract
Pot marigold (Calendula officinalis L.) is a medicinal and ornamental plant. The effect of different concentrations of chlormequat (cycocel), and daminozide, two plant growth retardant, on plant height, flowering, the content of essence and some other traits in pot marigold (Calendula officinalis) was ...
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Pot marigold (Calendula officinalis L.) is a medicinal and ornamental plant. The effect of different concentrations of chlormequat (cycocel), and daminozide, two plant growth retardant, on plant height, flowering, the content of essence and some other traits in pot marigold (Calendula officinalis) was assessed. Plant growth retardants are commonly applied to limit stem elongation and produce a more compact plant. The experiment was done as a factorial in randomized completely blocks design (R.C.B.D.) with 16 treatments and 3 replications in Rasht. Cycocel at 4 concentrations (0, 500, 1000 and 1500 mg/L) and daminozide at 4 concentrations (0, 1500, 3000 and 4500 mg/L) were used. Investigated characteristics were plant height, leaf number, flower number, flowering time, fresh weight, dry matter, the content of essence and carotenoid in flowers. Based on analysis of variance (ANOVA), the effect of different treatments and their interaction on most traits was significant at 0.05 level of probability. The minimum height (24 cm/plant) in treatment of 500 mg/L cycocel + 3000 mg/L daminozide, the largest number of flowers (4.66 flowers/plant) in treatment of 1000 mg/L cycocel + 4500 mg/L daminozide and most essence content (0.154 mg/100 g) in treatment of 4500 mg/L daminozide without cycocel were obtained.